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High-resolution (80um isotropic) contrast-enhanced diffusion tensor data was acquired from six background control (C3HeB) and six dysmyelinating shiverer (C3Fe.SWV shi/shi) mouse brains. The data consists of nominally unweighted and diffusion weighted images with optimized icosahedral sampling.
Description
Animal Protocol — The brains of congenic male homozygous shiverer mutants (C3Fe.SWV Mbpshi/Mbpshi, Jackson Laboratories, mean age at fixation = 6.0 +- 0.2 weeks, n = 6) and control males with the same background as the shiverers (C3HeB/FeJ, Jackson Laboratories, mean age at fixation 6.9 +- 0.2 weeks, n = 6) were studied using diffusion tensor imaging. Mice were anesthetized deeply using 2.5% Avertin (0.017ml/g body weight). The mouse was then fixed by transcardiac perfusion using 30ml of room temperature heparinized phosphate buffered saline followed by 30ml of room temperature 4% paraformaldehyde (PFA). All experiments were performed in accordance with protocols approved by the Institutional Animal Care and Use Committee of the California Institute of Technology. After death, the head was removed and rocked in 4% PFA overnight at 4C. The skin, lower jaw, ears and cartilaginous nose tip were removed and the head rocked in 50ml 0.01% sodium azide in PBS for 7.0 +- 0.1 days (mean +- sd) at 4C. The head was then transferred to a 5mM solution of gadoteridol (Prohance, Bracco Diagnostics Inc, Princeton NJ) and 0.01% sodium azide in PBS and rocked for 13.5 +- 1.9 days at 4C prior to MR imaging. All brains were equilibrated at room temperature for 8.5 +- 3.0 hours immediately prior to imaging at 20C. In four control and four shiverer brains, DTI acquisitions were repeated to address B1 homogeneity concerns and the second dataset used in the results analysis. The additional time spent by these brains in 5mM gadoteridol is included in the quoted time intervals above. The repeated brains also spent an additional 6.8 +- 0.1 hours equilibrating to room temperature prior to imaging.
Magnetic Resonance Imaging — All images were acquired using a vertical bore 11.7 Tesla Bruker Avance DRX500 system (Bruker Biospin, Germany) equipped with a Micro2.5 imaging gradient set capable of a peak gradient strength of 1T/m and a maximum slew rate of 12.5kT/m/s. The intact head was secured in a Teflon holder and submerged in a perfluoropolyether (Fomblin, Solvay Solexis, Inc, Thorofare, NJ) within a 50ml vial and imaged using a 35mm birdcage transmit/receive volume resonator. The ambient bore temperature was maintained at 20C by thermostatically controlled airflow. Optimized second order shimming was achieved across the whole sample using the Bruker implementation of Fastmap 1. Diffusion weighted images were acquired using a conventional pulsed-gradient spin echo (PGSE) sequence (TR/TE = 150ms/11.6ms, 256 x 150 x 130 matrix, 19.2mm x 15mm x 12mm FOV, 80_m isotropic voxel size, 1 average, _ = 3ms, _ = 5ms, Gd = 750mT/m, nominal b-factor = 1450 s/mm2). An optimized six point icosahedral encoding scheme 2 was used for diffusion weighted acquisitions with a single un-weighted reference image for a total imaging time of 6 hours.
Figure: Students t-statistic map for the group mean comparison between the diffusion tensor trace in wild-type and shiverer mutant mice overlaid on the edge-detected mean iDWI image for the wild-type group.
Accession Number
TBD
Citations to Include
Tyszka, J.M., Readhead, C., Bearer, E.L., Pautler, R.G. & Jacobs, R.E. Statistical diffusion tensor histology reveals regional dysmyelination effects in the shiverer mouse mutant. Neuroimage (2005).
Contributors
J. Michael Tyszka1, Carol Readhead1, Elaine L. Bearer1,2, Robia G. Pautler1,3 and Russell E. Jacobs1
- Biological Imaging Center, Division of Biology, California Institute of Technology, Pasadena, CA
- Department of Pathology and Medicine, Brown University, Providence, RI
- Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX
Technical Contact
J. Michael Tyszka, Ph.D.
Director, MR Physics
Caltech Brain Imaging Center
Email: jmt *AT* caltech.edu
Acknowledgements
The authors wish to thank Tim Hiltner for mouse perfusions and brain preparation, Natasha Kovacevic, Josette Chen and Mark Henkelmann for invaluable discussions regarding image coregistration and tissue fixation. This work was funded in part by the Human Brain Project (EB00232) with contributions from the National Institute of Biomedical Imaging and Bioengineering and the National Institute of Mental Health, the NCRR (RR13625), and MH61223.
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